--- type: "Chapter" knowledge_type: "chapter" entity_type: "chapter" id: "66def6593f8b4e9e69bd7ae7" title: "Plant Tissue Culture" slug: "plant-tissue-culture" chapter_number_in_book: "7" board: "CBSE" curriculum: "CBSE" class: "Class 12" subject: "Biotechnology" book: "Biotechnology" canonical_url: "https://www.edzy.ai/cbse-class-12-biotechnology-plant-tissue-culture" markdown_url: "https://www.edzy.ai/okf/chapter/cbse-class-12-biotechnology-plant-tissue-culture.md" source_type: "examSubjectBookChapter" source_id: "66def6593f8b4e9e69bd7ae7" source: "Edzy" source_pdf: "https://edzy-ai.s3.ap-south-1.amazonaws.com/edzy-express-ts/988b82d7-3428-4672-84ed-1d4f818c934e.pdf" book_page_start: "167" book_page_end: "184" book_page_count: "18" starts_on_page: "167" ends_on_page: "184" version: "1" last_updated: "2026-06-25" svg_icon: "https://edzy-ai.s3.ap-south-1.amazonaws.com/academic/class-12/66def6593f8b4e9e69bd7ae7.svg" book_svg_icon: "https://edzy-ai.s3.ap-south-1.amazonaws.com/academic/class-12/66ae541b191fdf5766655c83.svg" subject_svg_icon: "https://edzy-ai.s3.ap-south-1.amazonaws.com/edzy-express-ts/ac3027ed-1579-42c6-9bcf-af370d0cfef7.svg" color_name: "Aqua Emerald" color_light_foreground: "#00B3A6" color_light_background: "#D9FFFC" color_dark_foreground: "#00B3A6" color_dark_background: "#004B45" --- # Plant Tissue Culture Plant Tissue Culture is chapter 7 in Biotechnology, a CBSE Class 12 book for the subject Biotechnology on Edzy. ## Knowledge Snapshot | Field | Details | | :--- | :--- | | Board | CBSE | | Curriculum | CBSE | | Class | Class 12 | | Subject | Biotechnology | | Book | Biotechnology | | Chapter | Plant Tissue Culture | | Chapter Number In Book | 7 | | Chapter Slug | plant-tissue-culture | | Pages | 167-184 | | Page Count | 18 | | Source PDF | https://edzy-ai.s3.ap-south-1.amazonaws.com/edzy-express-ts/988b82d7-3428-4672-84ed-1d4f818c934e.pdf | | Last Updated | 2026-06-25 | ## Chapter Overview Plant tissue culture (PTC) refers to the cultivation of undifferentiated plant cells, tissues, or organs on synthetic media under aseptic environments and suitable controlled physical conditions. It is based on the unique characteristic of a plant cell called totipotency, which is the ability of a vegetative cell to divide and differentiate into specialized cells or regenerate into a whole plant. The chapter discusses the historical perspectives of PTC, key techniques involved, the rationale behind nutrient media, various culture types, and the applications of plant cell and tissue culture. ## Topic Inventory | No. | Topic ID | Topic | | :--- | :--- | :--- | | 1 | 69aa796fa243cecf9627b293 | Historical Perspective | | 2 | 69aa796fa243cecf9627b295 | Cell and Tissue Culture Techniques | | 3 | 69aa796fa243cecf9627b297 | Nutrient Media | | 4 | 69aa796fa243cecf9627b299 | Culture Types | | 5 | 69aa796fa243cecf9627b29b | Applications of Plant Cell and Tissue Culture | ## Topic-Wise Extracted Content ### Historical Perspective In the 19th century, German scientists Theodor Schwann and Matthias Schleiden highlighted the importance of the cell as the basic unit of life. Gottlieb Haberlandt pioneered the field of PTC in the 1890s and proposed principles for plant cell growth on nutrient media, earning him the title 'Father of Plant Tissue Culture'. ### Cell and Tissue Culture Techniques During the early 20th century, the ability to culture isolated plant tissues grew. The techniques refined vitamin and natural auxin use in cultures. Significant discoveries included the introduction of coconut water, adenine, and kinetin to enhance plant growth in cultures. ### Nutrient Media Murashige and Skoog (MS) medium, developed in 1962, became widely utilized for plant tissue cultures, allowing for successful initiation across diverse plant species. The hormonal balance of auxins and cytokinins is crucial for root and shoot differentiation in cultured cells. ### Culture Types Culture types generally harness the totipotency of plant cells, integrating various explants like root and shoot tips and displaying diverse outcomes depending on growth hormones present. ### Applications of Plant Cell and Tissue Culture This technology finds applications in commercial plant production, genetic engineering, and creating transgenic plants, facilitating advancements such as the development of 'Golden Rice' and other genetically modified organisms. ## Chapter Summary This chapter explores the significant developments in plant tissue culture, illustrating its fundamentals, historical milestones, and practical applications. Important discoveries upon which PTC has evolved are emphasized, providing insight into how these methodologies have shaped modern biotechnology. ## Key Ideas - Plant tissue culture relies on the principle of totipotency. - Gottlieb Haberlandt is recognized as the 'Father of Plant Tissue Culture'. - The Murashige and Skoog medium remains widely utilized for plant tissue culture. - Balanced growth hormones determine morphological outcomes in cultured cells. - PTC applications include commercial production and genetic modifications. ## Important Chapter Facts - The chapter is Plant Tissue Culture. - The chapter number in the book is 7. - The subject is Biotechnology. - The book is Biotechnology. - The class is Class 12. - The source PDF is [available here](https://edzy-ai.s3.ap-south-1.amazonaws.com/edzy-express-ts/988b82d7-3428-4672-84ed-1d4f818c934e.pdf). - The chapter covers pages 167-184. - The chapter page count is 18. - The topic list contains the supplied topic entries. ## Related OKF Entities | Entity Type | Title | URL | | :--- | :--- | :--- | | Chapter Canonical Page | Plant Tissue Culture | https://www.edzy.ai/cbse-class-12-biotechnology-plant-tissue-culture | | Chapter Markdown | Plant Tissue Culture | https://www.edzy.ai/okf/chapter/cbse-class-12-biotechnology-plant-tissue-culture.md | | Source PDF | Plant Tissue Culture | https://edzy-ai.s3.ap-south-1.amazonaws.com/edzy-express-ts/988b82d7-3428-4672-84ed-1d4f818c934e.pdf | ## Source Attribution | Field | Value | | :--- | :--- | | Source | Edzy | | Reference Type | examSubjectBookChapter | | Reference ID | 66def6593f8b4e9e69bd7ae7 | | Chapter ID | 66def6593f8b4e9e69bd7ae7 | | Canonical URL | https://www.edzy.ai/cbse-class-12-biotechnology-plant-tissue-culture | | Markdown URL | https://www.edzy.ai/okf/chapter/cbse-class-12-biotechnology-plant-tissue-culture.md | | Source PDF | https://edzy-ai.s3.ap-south-1.amazonaws.com/edzy-express-ts/988b82d7-3428-4672-84ed-1d4f818c934e.pdf | | Last Updated | 2026-06-25 |