Flash Cards: Applications of Recombinant DNA Technology
This chapter discusses various applications of recombinant DNA technology, highlighting its significance in modern biotechnology.
Structured practice
Applications of Recombinant DNA Technology - Flash Cards
These flash cards cover important concepts from Applications of Recombinant DNA Technology in Biotechnology for Class 12 (Biotechnology).
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What is recombinant DNA technology?
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Recombinant DNA technology is a set of techniques used to combine genetic material from multiple sources into a single DNA molecule to create new genetic combinations.
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What is DNA fingerprinting used for?
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DNA fingerprinting is used for identifying individuals based on unique patterns in their DNA, particularly in paternity testing and forensic science.
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What does VNTR stand for?
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VNTR stands for Variable Number Tandem Repeat, which refers to short sequences of nucleotides that are repeated in the genome and vary in number between individuals.
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What is the difference between microsatellites and minisatellites?
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Microsatellites are repeat sequences of 1 to 9 base pairs, while minisatellites are repeat sequences of 10 to 100 base pairs.
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Why is PCR used in DNA fingerprinting?
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Polymerase Chain Reaction (PCR) is used to amplify DNA, increasing the sensitivity of the fingerprinting technique, especially with small DNA samples.
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What does RFLP stand for and what is its role?
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RFLP stands for Restriction Fragment Length Polymorphism, which is a technique used to analyze DNA samples by cutting them with restriction enzymes to produce unique fragment patterns.
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What is Southern hybridization?
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Southern hybridization is a technique used to detect specific DNA sequences by hybridizing a DNA probe to fragments separated on a gel.
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Name one application of DNA fingerprinting.
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One application of DNA fingerprinting is in forensic science to identify suspects based on DNA extracted from crime scenes.
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How does DNA fingerprinting ascertain paternity?
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DNA fingerprinting analyzes the VNTR patterns of a child and compares them with those of the parents to confirm biological relationships.
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What is one use of DNA fingerprinting in evolutionary biology?
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DNA fingerprinting can compare DNA from fossils to modern species, providing insights into evolutionary relationships.
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What is a common mistake students make in DNA fingerprinting?
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A common mistake is confusing the DNA patterns of a parent with those of siblings, not recognizing that patterns can differ.
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What are recombinant vaccines?
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Recombinant vaccines are produced using recombinant DNA technology to prompt an immune response without using live pathogens.
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What is gene therapy?
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Gene therapy involves altering the genes inside an individual's cells to treat or prevent disease, often using recombinant DNA technology.
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What are transgenic organisms?
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Transgenic organisms are those that have been genetically modified to carry a gene from an unrelated organism, enhancing traits or functions.
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From where can DNA be isolated for fingerprinting?
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DNA can be isolated from samples such as blood, hair, skin, semen, and buccal swabs.
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What is the purpose of gel electrophoresis in DNA analysis?
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Gel electrophoresis separates DNA fragments based on size, allowing for analysis and comparison of DNA profiles.
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What is polymorphism in DNA?
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Polymorphism refers to the variations in DNA sequences that can lead to differences between individuals, essential for DNA fingerprinting.
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What is a proposed use for DNA profiles?
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DNA profiles are proposed to be used as genetic barcodes for personal identification, enhancing security and accuracy.
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What does 'genetic polymorphism' mean?
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Genetic polymorphism refers to the presence of two or more variations (alleles) in a population, leading to genetic diversity.