Question Bank: Host-Vector System

What are the two key components of recombinant DNA technology?

Which of the following is the most commonly used prokaryotic host for gene cloning?

What is the primary function of a vector in recombinant DNA technology?

Which of the following features is NOT typical of a good vector?

What type of host is yeast when used in recombinant DNA technology?

What is the role of restriction enzymes in recombinant DNA technology?

Which vector is specifically designed for the expression of proteins in a host?

Why is plasmid considered a popular vector for gene cloning?

What characteristic of E. coli makes it suitable for cloning?

Which of the following vectors can be used in both prokaryotic and eukaryotic systems?

Which of the following statements best describes a capability required of a host in recombinant DNA technology?

What is a major advantage of using Bacillus subtilis as a host?

What factor does a vector's 'origin of replication' provide?

Why must a host not degrade recombinant DNA?

What is an expression vector primarily designed for?

What are R-plasmids primarily known for?

Which structure is formed by plasmid transfer genes to connect to other bacterial cells?

What is the main characteristic of high copy plasmids?

Col plasmids are primarily responsible for the production of which of the following?

What type of vector is characterized by having features of both plasmids and lambda phage vectors?

During the life cycle of the M13 bacteriophage, what is the double-stranded replicative form (RF) primarily used for?

Which of the following statements about episomes is true?

What aspect of a vector is important for screening transformed cells?

Which of the following is NOT a common feature of vectors used in genetic engineering?

What defines a low copy plasmid?

Why are plasmids considered useful tools in biotechnological applications?

Which mechanism is NOT used by F-plasmids to transfer genetic material?

Why is it essential for vectors to have an origin of replication?

When producing a recombinant plasmid, why is it necessary to use restriction enzymes?

Which characteristic is often associated with the plasmids that carry toxin genes?

What is the role of selectable markers in vectors?

What is the primary function of a vector in recombinant DNA technology?

Which of the following is a characteristic of plasmid vectors?

Which type of vector is specifically designed for the expression of cloned genes?

Which of the following is a key advantage of using a bacteriophage as a vector?

In recombinant DNA technology, what is the purpose of a selectable marker within a vector?

What type of vector combines features of plasmids and bacteriophages?

What characteristic distinguishes shuttle vectors?

Which of the following is NOT a feature of an ideal cloning vector?

What is a primary disadvantage of using BACs (Bacterial Artificial Chromosomes) as vectors?

Why are eukaryotic expression vectors needed?

What role do restriction enzymes play in the use of vectors?

What is the purpose of using a low copy number vector?

Which is a common misconception about vectors in recombinant DNA technology?

How would you classify a cosmid based on its properties?

What feature is essential for a vector to function in both prokaryotes and eukaryotes?

Which of the following organisms is the most commonly used eukaryotic host in recombinant DNA technology?

What is the primary role of vectors in recombinant DNA technology?

What characteristic must a suitable vector possess?

Which type of vector allows cloning of very large DNA fragments (up to 1 million base pairs)?

What is a primary advantage of using yeast as a host organism in recombinant DNA technology?

Which of the following is NOT considered a characteristic of an effective vector?

In yeast artificial chromosomes, what role do telomeres play?

What is the purpose of a selectable marker in a vector?

Which of the following cloning vectors is required for incorporating DNA fragments over 10 kb in eukaryotic systems?

What defines a single copy plasmid's replication strategy?

Which of the following vectors is a hybrid of plasmid and bacteriophage?

Which essential feature must an expression vector possess?

Why are yeast artificial chromosomes used in cloning?

What is a key feature of the pBR322 plasmid?

What role does the autonomously replicating sequence (ARS) play in yeast vectors?

What is the primary function of a shuttle vector?

Which of the following is not a characteristic of shuttle vectors?

What is a primary benefit of using shuttle vectors in biotechnology?

Which two systems do shuttle vectors commonly replicate in?

An example of a prokaryotic cell often used with shuttle vectors is?

What is typically included in the construction of a shuttle vector?

Shuttle vectors are useful for studying which type of gene expression?

Which of the following is an example of a eukaryotic cell that can be used with shuttle vectors?

Shuttle vectors differ from standard plasmids in that they can?

Why might researchers choose to use shuttle vectors in their experiments?

In a shuttle vector, how is the correct origin of replication selected in a specific host?

What critical role does the selectable marker serve in a shuttle vector?

Which of the following types of genes can be studied using shuttle vectors?

What is the primary function of a vector in recombinant DNA technology?

Which of the following is a commonly used prokaryotic host for gene cloning?

What is one characteristic that an expression vector must have?

What is the role of selectable markers in vectors?

Which vector type can accommodate the largest DNA inserts?

What is a shuttle vector?

What is the general role of an inducible promoter in expression vectors?

Which size of DNA insert can a bacteriophage vector accommodate?

What is a disadvantage of using prokaryotic hosts for eukaryotic gene expression?

Which of the following is NOT typically found in an expression vector?

What is the function of the ligation process in rDNA technology?

What is a common advantage of using yeast as a eukaryotic host?

Which structure of a plasmid is crucial for its replication?

In recombinant DNA technology, what is the purpose of the term 'heterologous expression'?

What will likely happen if a recombinant plasmid is not properly maintained within a host cell?