Flash Cards: Genome Technology and Engineering

The genome is the complete genetic information present in the cell of an organism, including DNA in prokaryotes and the DNA in eukaryotic chromosomes.

Genetic mapping estimates distances between genetic loci based on the frequency of recombination and crossover events.

RFLP is a mapping technique that involves cutting DNA with restriction enzymes and analyzing the resulting fragment patterns.

Sequence Tagged Sites are unique DNA sequences used as landmarks in physical mapping of genomes, identified by PCR.

First generation sequencing is a multistep procedure involving DNA fragment separation, restriction digestion, and cloning for sequencing.

NGS uses massively parallel sequencing technology to read millions of DNA fragments simultaneously, enabling rapid sequencing of whole genomes.

WGS determines the complete DNA sequence of an organism's genome, useful for understanding genetic disorders and inheritance.

Metagenomics studies the collective genetic material from a community of organisms in their natural environment, without culturing them.

CRISPR-Cas9 is a genome editing technique that uses guide RNA and Cas9 enzyme to create double-strand breaks for precise genetic modifications.

Transposons, or jumping genes, are DNA sequences that can move within a genome, often used for gene knockout and insertion.

Functional genomics aims to understand how gene information is executed physiologically within the organism.

Comparative genomics focuses on comparing genes and genomes across species to identify conserved and unique genes.

High-throughput sequencing allows rapid sequencing of large amounts of DNA, significantly speeding up genomic analysis.

Protein engineering involves modifying protein structures to enhance their properties for research and therapeutic applications.

CRISPR can be used for gene editing, developing disease-resistant crops, and in gene therapy for treating genetic disorders.

RNA-Seq is a next-generation sequencing technique used to analyze the transcriptome and gene expression profiles of organisms.

Cloning vectors are DNA molecules used to transport and replicate foreign DNA segments in host cells during cloning.

Gene therapy is a technique aimed at correcting defective genes responsible for disease development, often using viral vectors.